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Peptide Calculator

Peptide

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Results

Number of Residues:
1-Letter Code:
3-Letter Code:

Molecular weight (Mr):	g/mol
Isoelectric point:
Net charge at pH 7.0:
Average hydrophilicity:
Ratio of hydrophilic residues / total number of residues:	%

Net Charge	Hydrophilicity
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ChinaPeptides Peptide Calculator Manual

Enter Peptide Sequence:

1. Input Sequence

You can input the peptide sequence in 1-letter Code or/and 3-letter Code.

2. Modification on terminus.

Modification of Acetylation, Biotinlytion and Pyr are available to N-terminus, Amidation is available to C-terminus.

Result Analysis:

1. Molecule Weight

Calculating your peptide and get a precise peptide molecular weight in seconds.

2. PI

The isoelectric point, PI, is the PH at which the Net Charge of the peptide is zero. The isoelectric point is calculated by approximation(accuracy±0.01).

3. Net charge at PH 7.0

On the “Net Charge” graph, you can view the peptide net charge status under different PH conditions. * Lehninger(1982) Principles of Biochemistry

4. Average hydrophilicity

From average hydrophilicity value, we can predict the peptide solubility in water.

Average Hydrophilicity Value is 2～3：	Peptide have a good solubility in water, it is also easy to absorb the moisture from the air, To solve this problem, ChinaPeptides has built a GMP grade packing room, in which the moisture of the air is strictly controlled, all the packing works are finished in this room, and a desiccant is also been put in the peptide express package.
Average Hydrophilicity Value is 0～2：	Generally, peptide with this Hydrophilicity grade always have a good solubility in water. No cosolvent needed.
Average Hydrophilicity Value is 0：	This kind of peptide has a poor solubility in water, there are many ways to increase the peptide solubility. 1, adjusting the PH. 2, Apply Ultrasonic 3, adjusting the temperature(≤30℃ ), 4，Apply cosolvent(such as DMSO, DMF), 5, using 10% acetic acid to dissolve the alkaline peptide, 10% ammonium acid carbonate to dissolve the acidic peptide.
Average Hydrophilicity Value is -2～0：	Peptide has a poor solubility in water, you can apply the 5 methods above to dissolve your peptide, or you can add hydrophilic amino acids into the peptide sequence.
Average Hydrophilicity Value is -2～-3：	Peptide in this hydrophilicity grade always has a poor solubility in water. It is also hard to purify this kind of peptide, for this reason, it’s very tough to have a good peptide purity. In this case, we usually advise our customer redesigning the peptide sequence or insert some hydrophilic amino acids into the peptide sequence.

* Hopp & Woods Note: Secondary structure, Conjugation, Modification are also affects the solubility of the peptide.

5. The ratio of hydrophilic value

The ratio of hydrophilic residues to total number of amino acids is reported in %.

For any question, please contact us at:
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