Prokaryotic proteins purification services

prokaryotic expression is the method of inserting cloned gene into suitable vector and introducing into E. coli for expression of large amounts of protein.prokaryotic expression system is by far the more mature and reliable protein expression system which rapidly express and purify a variety of protein sources. Prepared recombinant protein can be used for drug screening, structural biology, cell biology, proteomics and other fields of biomedical.Based on different expression requirements, such as volume of expression, viability of targeted protein and different purification method, we choose proper expression system and corresponding expression strategy. We make use of our mature and steady protein purification system to optimize expression terms and purify targeted protein.

Service Number: QY-4003
Service Items:
Select of plasmid transformation and expression strain
Cultivate Transformed bacteria flask and Inducible expression
Purify of Targeted proteins
Detect expression and purification through SDS-PAGE
Final material delivery:
Initial culture conditions and reasonable purification process; purified protein (1mg, more than 85% purity)
SDS-PAGE protein electrophoresis results and detailed experimental records
Product Report (including relevant data, SDS-PAGE and plasmid sequencing results, etc.)
Sample requirement:
Provide cloned prokaryotic expression vector or plasmid or strain in cloning vector
Provide relevant information and Nucleic acid/protein sequence,use bioinformatics methods to analyze and make concrete and feasible program of the experiment
Attention:
1.Expression vector provided by client shall be transformed and expressed, proving with the expression of the target protein, plasmid ≥1ug
2.Expression protein should be equipped with His and GST label.