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Mammalian cell protein expression
Our company has Successfully builded a complete variety of protein expression technology platform: including the prokaryotic protein expression and purification, expression and purification of yeastprotein,expression and purification of protein which from insect cells, expression and purification of proteins from mammalian cells. According to customer needs, we can offer one-stop services which comprise gene synthesis, vector, gene expression, protein purification, detection and other . Our company has established a strict quality control system and a set of perfect customer service processes, confidentiality system to ensure that customers would get the quality products and more services Our team has very rich experience in optimizing gene codon and expression vector design and construction, integration of objects and tags selection, specific protein purification system selection, protein production process optimization and so on. we canmeet the needs of individual customers.
Mammalian cell protein expression
Mammalian cell protein expression is suitable for large-scale and fast preparation of protein, we provide the services contained construction of expression vector, transient transfectionand detection of protein expression and purification ofprotein and so on. It can be divided into transient expression, stable expression, stable expression of cell lines.
Transient expression of mammalian cell protein service TGE (HEK293 or CHO cells)
We can provide the amount of Mammalian cell protein from milligrams to grams.Fast transfection can be completed generally in 10 days, without the genetic selection ofDNA from plasmid. Our transient expression using suspended cells, can easily express ml to 100L protein. When construction of the expression vector was completed, we can deliver the product to the customer(the cell culture fluid collection or recombinant protein). If the customer needs, the target gene could be cloned into a ly efficient expression vector designed for the TGE system.
1, the cloning of the coding sequence of target protein to the mammalian transient expression vector;
2, the preparation of plasmid which can be used for transfection of mammalian cells;
3, assessment and optimization of mammalian cell transfection, recombinant protein expression ;
4, expression and purification of small scale protein (500 ml Culture), detection of protein ;
5,expression and purification of large-scale protein.
Stable expression technology service of mammalian cell protein
Throughout the experimental procedure, from transfection, screening and separation, to evaluating the production capacity and adaptability of primary clones, our company provide long-term stable and adjustable scale protein production. According to customer demand. We also have the ability of comprehensive genetic research to ensure the stability of protein production.
1, the cloning of the coding sequence of target protein to the mammalian transient expression vector;
2, the preparation of plasmid which can be used for transfection of mammalian cells;
3, assessment and optimization of mammalian cell transfection, recombinant protein expression ;
4. Monoclonal screening and expression of recombinant protein;
5, expression and purification of small scale protein (500 ml Culture), detection of protein ;
6,expression and purification of large-scale protein.
Construction of stable expression cell lines for scientific research
Our company provide synthetic gene, cloning, vector design construction, protein purification and characterization, and new drug screening a station type service.We can build and screening cell line which would meet your needs , explore faster better experimental solutions, help you save a lot of time in the late of the project .
1, customers can choose the cell by youself orbuy from our company;
2, we can provide the cloning of coding sequence of the target protein to the appropriate vector ;
3, the preparation of plasmid which can be used for transfection of mammalian cells;
4, transfection of mammalian cell , screening stably transfected cells;
5, assessment. of monoclonal screening (optional) andexpression of recombinant protein.
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