An immunoassay is a biochemical test that measures the presence or concentration of a substance in solutions that frequently contain a complex mixture of substances. Such assays are based on the unique ability of an antibody to bind with specificity to one or a very limited group of molecules. A molecule that binds to an antibody is called an antigen. Immunoassays can be carried out for either member of an antigen/antibody pair. The specific and effective antibody is a key to a successful immunoassay.Antibody purification is performed to concentrate and enrich antigen-specific antibodies and lower background by removing any non-specific proteins. Antibody labeling can amplify signal and increase sensitivity. There are many immunoassay techniques such as ELISA、WB、IP and IHC etc.

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