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Mass spectrometry is the analysis techniques, ionization the sample nto a gaseous mixture of ion, and separation according to the load ratio (m/z)./p>

Mass spectrometer is composed of the following sections

Ion source is the devices cause the atoms or molecules of analysis samples into charged particles (ions), and to accelerate the ions into the analyzer, according to the different methods of ionization, biomacromolecule analysis commonly used are electrospray Ionization and matrix Assisted Laser Desorption.

According to the quality analyzer, the following several kinds are common quadrupole mass spectrometer (Q), the ion TRAP mass spectrometer (Ions TRAP), the time of flight mass spectrometer (TOF), fourier transform ion cyclotron resonance mass spectrometer (FT - ICRMS).

Molecular weight is a key characteristic parameters of protein, but also the more basic physical and chemical properties of parameters of the organic compounds. The molecular weight correctly or not often represents the organic compounds and macromolecular structure correctly or not. Molecular weight is also the primary parameters such as peptides, proteins, along with the advancement of genome and proteome research, protein molecular weight determination of scientific research work has received more and more attention. The following methods determinate the protein molecular weight accurate by ESI - TOF, used Mass Spectrometer TripleTOF 5600 Manufacturer AB Sciex Instruments, also combination with other detection methods of molecular weight Mass spectrometry, the result is further confirmed.