Name
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Α-Factor Mating Pheromone, Yeast
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Other Name
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pheromone moth; mating animals
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Sequence (Single letter abbreviations)
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WHWLQLKPGQPMY
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Sequence(Three letter abbreviations)
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{TRP}{HIS}{TRP}{LEU}{GLN}{LEU}{LYS}{PRO}{GLY}{GLN}{PRO}{MET}{TYR}
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Basic description
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The alpha factor pheromone arrests yeast in the G1 phase of their cell cycle. Alpha Factor Mating Pheromone induces the expression of mating genes, changes in nuclear architecture, and polarzes growth toward the mating partner. STE2 encodes the alpha factor pheromone receptor (a GPCR) found on mating-type-A cells in yeast.
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Solubility
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Soluble in water
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The molecular weight
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1683.980
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Chemical formula
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C82H114N20O17S
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The purity
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> 95%
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Storage conditions
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Store this peptide in its dry form at -20°C. For results, rehydrate the peptide immediately before use. Do not re-freeze any unused portions.
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Annotation
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Simply thaw and use directly. We recommend using it at concentrations ranging from 5 µM (bar1 D) to 100 µM (BAR1).
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Documents
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Figures
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Reference
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Busnelli S., et al. Snf1/AMPK promotes SBF and MBF-dependent transcription in budding yeast. Biochim Biophys Acta. 2013 Dec;1833(12):3254-64.
Boeckmann L., et al. Phosphorylation of centromeric histone H3 variant regulates chromosome segregation in S. cerevisiae. Mol Biol Cell. 2013 Jun;24(12):2034-44.
Yang Z., et al. Heterologous gln/asn-rich proteins impede the propagation of yeast prions by altering chaperone availability. PLoS Genet. 2013 Jan;9(1):e1003236.
Lickwar CR., et al. Genome-wide measurement of protein-DNA binding dynamics using competition ChIP. Nat Protoc. 2013 Jun;8(7):1337-53.
Fong CM., et al. The Saccharomyces cerevisiae F-Box Protein Dia2 Is a Mediator of S-Phase Checkpoint Recovery from DNA Damage. Genetics. 2013 Feb;193(2):483 - 99.
Peter Tessarzet al. Glutamine methylation in histone H2A is an RNA-polymerase-I-dedicated modification. Nature 2013 Dec;
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