Name
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Hexa-His
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Other Name
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His-taghis tagged; epitope tag
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Sequence (Single letter abbreviations)
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HHHHHH
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Sequence(Three letter abbreviations)
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{HIS}{HIS}{HIS}{HIS}{HIS}{HIS}
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Basic description
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The amino acid sequence consisting of 6 His residues in a row, called His-Tag, would act as a metal binding site for a recombinant protein. His-Tag sequence can be placed on the N- or -terminal of a target protein by using vectors from various commercial molecular biology companies. And this Tag is often followed by a cleavage site for a specific protease - for example Leu-Val-Arg-Gly-Ser peptide sequence is recognized and cleaved by the protease known as thrombin.
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The molecular weight
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840.850
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Chemical formula
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C36H44N18O7
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The purity
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> 95%
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Storage conditions
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Store at -20°C. Keep tightly closed. Store in a cool dry place
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Annotation
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Documents
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Figures
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Reference
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Hammarstrom M, et al. Effect of N-terminal solubility enhancing fusion proteins on yield of purified target protein. J. Struct. Funct. Genomics. Jul 2006
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