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Your location:Home>> Products >> ELISA

强耀生物科技 Mouse gp130 ELISA Kit/小鼠糖蛋白130试剂盒
Name Mouse gp130 (Glucoprotein 130) ELISA Kit。
Briefly steps: 1. In each hole adding standard or sample 100 mu L, 37 ℃ for 90 minutes incubation
2. Add in 100 mu L biotin antibody working liquid, 37 ℃ incubation 60 minutes
3. Wash three times
4. Add in 100 mu L enzyme combined with the working liquid, 37 ℃ incubation for 30 minutes
5. Washing five times
6. Join the 90 mu L substrate solution, 37 ℃ incubation for about 15 minutes
7. Add 50 mu L terminated liquid, at 450 nm wavelength measuring OD value immediately
8. The results calculated
Species Rat
Specifications 96T。
Detection range 15.625~1000pg/mL。
Sensitivity 9.375pg/mL。
Specificity No cross reaction system and other protein factors.
The period of validity 6 months(4℃);12 months(-20℃)。


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