名称
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MMP Substrate, Fluorogenic
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编码
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别名
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MMP Substrate, Fluorogenic
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纯度
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80%,90%,95%,98%,99%
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重量
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1mg,5mg,10mg,50mg,100mg,1g
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序列(单字母缩写)
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Dnp-PLGLWA-DArg-NH2
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序列(三字母缩写)
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DNP-Pro-Leu-Gly-Leu-Trp-Ala-DArg-NH2?(trifluoroacetate salt)
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基本描述
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Highly soluble fluorogenic matrix metalloproteinase (MMP) substrate with the following kcat/km values (at pH 7.6, 37°C): 619.0 M-1s-1?for MMP-2, 209.0 M-1s-1?for MMP-9, 40.0 M-1s-1?for MMP-3, 21.0 M-1s-1?for MMP-1. The fluorescence increase is measured using an wavelength of 340 nm and emission wavelength of 485 nm. The relative high emission wavelength of EDANS allows convenient measurement of MMP activity in complex biological media like synovial fluid and culture medium.
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溶解度
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分子量
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977.1
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化学式
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C45H64N14O11
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存储条件
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Store at -20°C. Keep tightly closed. Store in a cool dry place.
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注释
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Documents
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Figures
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Reference
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D.M. Bickett, et al., Anal. Biochem. 212, 58 (1993)
C.G. Knight, et al., FEBS Lett. 296, 263 (1992)
K. Darlak, et al., J. Biol. Chem. 265, 5199 (1990)
M.S. Stack, and R.D. Gray, J. Biol. Chem. 264, 4277 (1989)
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C端
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N端
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化学桥
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