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Protein expression in insect cells
Chinapeptides has successfully built a complete variety of protein expression technology platform, including prokaryotic, yeasty, entomic and mammalian protein expression and purification
According to customer needs, we offer gene synthesis, vector, gene expression, protein purification, detection and other one-stop service. In order to ensure the quality of protein purification, Chinapeptides establish a strict quality control system and a set of comprehensive customer service processe, to ensure the quality products and excellent service.
Our team has a very rich experience in protein gene codon optimization, vector construction design and expression, integration and label, specific protein purification systems optimization, to meet the needs of individual customers.
Insect Cell Protein Expression and Purification
Insect cell expression system is widely-used eukaryotic expression system that can express a variety of exogenous genes including fungi, plants, bacteria, virus genes.
Baculovirus is a type of double-stranded DNA that make insect cells as the natural host. Baculovirus is highly species-specific, and do not infect vertebrate, human, animal. More current research are about Autographa californica nuclear polyhedrosis (AcNPV), which host Spodoptera frugiperda.
sf9 cells, one of the host cell for baculovirus expression system. sf9 cells, kind of semi-adherent cell line, can be suspension culture or adherent culture with many many advantages。
1、 compared with other eukaryotic expression systems, it has high exogenous gene expression level, especially intracellular proteins. In cases,recombinant protein is soluble, correctly
folding have posttranslational modifications (such as glycosylation, etc.), biologically activity, easy separation and purification;
2、insect cells grown in suspension, which is easy for scaled-up cultures and large-scale expression of recombinant protein;
3、it is easy to express heterologous multimeric proteins. Insect cells can be infected through a variety of recombinant virus or can be implemented with a virus containing a plurality of expression cassettes;
4、security. Baculovirus infection do not include vertebrates.
Chinapeptides provides baculovirus expression vector, expression detecting and mass expression and purification of protein and other one-stop technical services.
1、coding sequence of target protein was cloned into the baculovirus transfer vector;
2、Preparation of a recombinant plasmid
3、transfected into insect cells, the virus modification;
4、viral titer test;
5、the recombinant protein expression verification and Optimization;
6、small-scale protein expression and purification (500 ml culture);
7、large-scale protein expression and purification (optional, suitable for biomedical research enterprise).
Technical services:
Baculovirus technical services
Protein expression optimization services
1、5-10ml recombinant baculovirus is provided by the customer;
2、insect cells are infected to obtain high titer virus;
3、optimization of expression conditions.
Optimization factors include:
Different insect cell lines;
Different expression time;
Different multiplicity of infection (MOI);
Virus titer test services;
Plaque assay titer test.
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