An immunoassay is a biochemical test that measures the presence or concentration of a substance in solutions that frequently contain a complex mixture of substances. Such assays are based on the unique ability of an antibody to bind with high specificity to one or a very limited group of molecules. A molecule that binds to an antibody is called an antigen. Immunoassays can be carried out for either member of an antigen/antibody pair. The high specific and effective antibody is a key to a successful immunoassay.Antibody purification is performed to concentrate and enrich antigen-specific antibodies and lower background by removing any non-specific proteins. Antibody labeling can amplify signal and increase sensitivity. There are many immunoassay techniques such as ELISA、WB、IP and IHC etc.
Chinapeptides has a leading antibody services technology, advanced equipments and professional technician, provides many services including antibody purification, antibody labeling and immunoassay techniques.
Service Code |
Service |
Brief Description |
Time |
QY-3001 |
Antibody Purification
（ammonium sulfate) |
1.ammonium sulfate precipitation of antisera（≤50 ml) 2.Detect concentration by UV absorption 3.Elisa |
1 week |
QY-3002 |
Antibody Purification
（ProteinA/G） |
1.antisera（≤50 ml) purification by proteinA/g 2.detect concentration by UV absorption 3.Elisa |
1 week |
QY-3003 |
Antibody Purification
（antigen affinity） |
1.antigen QC 2.antigen affinity resin prepare 3.antigen affinity purification（≤50 ml) 4.Detect concentration by UV absorption 5.Elisa |
2 week |
QY-3004 |
Antibody Label（HRP） |
1.antibody QC 2.antibody label with HRP 3.ELSIA |
1 week |
QY-3005 |
Antibody Label
（Biotin） |
1.antibody QC 2.antibody label with Biotin 3.label ratio test |
1 week |
QY-3006 |
Antibody Label（FITC） |
1.antibody QC 2.antibody label with FITC 3.label ratio test |
1 week |
QY-3007 |
ELISA |
Ag or Ab detect in indirect method,sandwich,or competitive methods |
1-2 weeks |
QY-3008 |
Western Blot |
Totol protein extract,SDS-PAGE,transfer,hybrid,detect&analysis |
2-3 weeks |
QY-3010 |
Immunohistochemistry
（IHC） |
Deparaffinization/Rehydration,Antigen Unmasking,Staining |
2-3 weeks |