Gene synthesis differ the oligonucleotide synthesis which is single-stranded oligonucleotides and the longest segment synthesized only about 100nt, means synthetic double-stranded DNA molecule technology in vitro, the length of the length of the synthetic range is 50 bp-12 kb. Gene synthesis is to use artificial methods of synthetic gene technology, one of the means of genetic gain, relative to obtained from the existing biological gene, gene synthesis without templates, and therefore not restricted by genetic source.

Gene synthesis definition

Humans’ first synthetic genes appeared in the 1960’s, synthetic gene is the main content of synthetic biology at present , through gene synthesis, genes does not exist in the nature can be obtained. This tech nology has opened up a new direction for the human biology. In the predictable future, gene synthesis will play a huge role in the life sciences, but in the new energy, new materials, artificial life, nucleic acid vaccine, the action of biological medicine, and other fields has been initial embodiment . Gene synthesis is also potentially could be developed into a biological weapons, and this possibility has become more prominent after several synthetic virus.

Gene synthesis has two ways, one is tailored to the gene synthesis company, the second is for a local gene synthesis. Because of gene synthesis technology has not yet been a unified method, the skills and experience in the process of gene synthesis has a decisive influence, so the gene synthesis is done in the hands of professionals, it also determines the way one is the main channel. Local gene synthesis generally need to refer to the academic articles, there are many aspects of this article. But mostly just the synthesis of individual genes, only have a limited reference value, unless in order to research the technology itself of gene synthesis. For local gene synthesis experimenter, useing the "kit" gene synthesis may be a good choice.

The advantages of gene synthesis

1 Synthesis period is short, can guarantee 100% of the sequence is correct.
2 Gene synthesis has greater flexibility, can modify enzyme loci of the genes and gene sequence, convenient downstream of the cloning and experiment.
3 The researchers can designed according to their will to get gene difficult to obtain even not exist in the nature.
4 Gene synthetic can proce codon optimization, so the gene expressed in all kinds of biological system can be well expressed.

The application of gene synthesis

1 Clone mouse antibodies or recombinant antibody.
2 Synthesis of different gene mutations, SNPS, or other mutations.
3 Design synthetic DNA vaccine.br/> 4 Synthesis a large number of cDNA for microchip.