Positional scanning is an important tool for peptide sequence optimization. Three position combinatorial scanning identifies amino acids of interest at three given positions and substitutes them with all other natural amino acids one at a time. Increases in activity will identify the preferred amino acid residues at these positions.
Special Instructions
Enter your original sequence below as well as the position of the three amino acids to be substituted, counting from the N-Terminus. A total of 20 x 20 x 20 = 8000 sequences will be generated.