Positional scanning is an important tool for peptide sequence optimization. This identifies an amino acid of interest at a single position and substitutes it with all other natural amino acids one at a time. Increases in activity will identify the preferred amino acid residues at this positions.
Special Instructions
Enter your target sequence below as well as the position(s) of the amino acid to be substituted, counting from the N-Terminus. A total of 20 sequences will be generated for each positional replacement.