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服务
产品  
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 Pep-1 (Uncapped)
| 名称
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Pep-1 (Uncapped)
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| 别名
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| 序列（单字母缩写）
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KETWWETWWTEWSQPKKKRKV
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| 序列（三字母缩写）
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{LYS}{GLU}{THR}{TRP}{TRP}{GLU}{THR}{TRP}{TRP}{THR}{GLU}{TRP}{SER}{GLN}{PRO}{LYS}{LYS}{LYS}{ARG}{LYS}{VAL}
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| 基本描述
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PEP-1 peptide, which has 21 amino acid residues, is a known carrier peptide that delivers full-length native proteins in vitro and in vivo. Pep-1 peptide, consists of three domains: (1) a hydrophobic tryptophan-rich motif containing five tryptophan residues (KETW WETWWTEW); (2) a hydrophilic lysine-rich domain (KKKRKV) derived from the nuclear localization sequence (NLS) of simian virus 40 (SV-40) large T antigen; and (3) a spacer domain (SQP), separating the two domains mentioned above, containing a proline residue, which improves the flexibility and the integrity of both the hydrophobic and the hydrophilic domains. In standard cell culture conditions, Pep-1 localizes rapidly, in <10 min, to the nucleus of human HS-68, murine NIH-3T3 fibroblasts, or Cos cells. Similar experiments, performed by incubating cells for 30 min at 4°C before transfection, yielded essentially the same result, indicating that Pep-1 internalization is independent of normal endocytosis.
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| 分子量
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2848.300
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| 化学式
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C136H195N35O33
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| 纯度
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> 95%
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| 存储条件
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Store at -20°C
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| 注释
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| Documents
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| Figures
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| Reference
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Weller K, et al. Biophysical and biological studies of end-group-modified derivatives of Pep-1. Biochemistry. Dec 2005; 44(48):1 5799-5811.
Henriques ST, et al. Re-evaluating the role of strongly charged sequences in amphipathic cell-penetrating peptides: a fluorescence study using Pep-1. FEBS. Lett. Aug 2005; 579(20): 4498-4502.
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