| Name
|
Uroguanylin, human
|
| Code
|
[154525-25-4]
|
| The alias
|
Uroguanylin, human
|
| Sequence (single letter abbreviation)
|
NDDCELCVNVACTGCL (C4&C12 bridge, C7&C15 bridge)
|
| Sequence (three-letter abbreviation)
|
H-Asn-Asp-Asp-Cys-Glu-Leu-Cys-Val-Asn-Val-Ala-Cys-Thr-Gly-Cys-Leu-OH (trifluoroacetate salt)(Cys4 and 12 bridge, Cys7 and 15 bridge)
|
| A basic description
|
A HCV protease substrate that incoporates an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm. As an internally quenced fluorogenic substrate, it can be used to monitor HCV NS3 protease activity.
|
| solubility
|
|
| The molecular weight
|
1667.9
|
| Chemical formula
|
C64H102N18O26S4
|
| The purity
|
80%，90%，95%，98%，99%
|
| Weight
|
1mg,5mg,10mg,50mg,100mg,1g
|
| Storage conditions
|
Store at -20°C. Keep tightly closed. Store in a cool dry place.
|
| Annotation
|
|
| Documents
|
|
| Figures
|
|
| Reference
|
T. Kita et al., Am. J. Physiol., 266, F342 (1994)
|
| The C-terminal
|
|
| The N-terminal
|
|
| Chemical bridge
|
(Cys4 and 12 bridge, Cys7 and 15 bridge)
|